mouse genome 430a 2.0 array  (Thermo Fisher)

Journal: Pathogens

Article Title: Ninoa T. cruzi Strain Modifies the Expression of microRNAs in Cardiac Tissue and Plasma During Chagas Disease Infection

doi: 10.3390/pathogens13121127

Figure Lengend Snippet: Bioinformatic analysis ( a ) Volcano plot showing underexpressed and overexpressed genes, with a fold-change less than −1 or greater than 1 and a p -value < 0.05. ( b ) Venn diagram illustrating the overlap of putative target genes for miR-1, miR-16, miR-208, and miR-208b from humans and mice, along with differentially expressed genes from Mouse Genome 430A 2.0 microarrays from the Affymetrix platform. ( c ) Enrichment pathways identified using ShinyGO v0.741 software and the number of participating genes in each pathway.

Article Snippet: Microarray data in the CEL format from the Mouse Genome 430A 2.0 Affymetrix platform were obtained from the Gene Expression Omnibus (GEO) database (accession number: GSE41089) ( https://www.ncbi.nlm.nih.gov/geo/ , accessed on 18 April 2023).

Techniques: Software

Journal: Cell reports

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner

doi: 10.1016/j.celrep.2024.114149

Figure Lengend Snippet: (A) Fold change in the expression of Thbs1 mRNA in soleus following 30 days of microgravity exposure (space flight) compared to control. Microarray dataset GEO: GSE80223 was analyzed from the Gene Expression Omnibus repository. Error bars denote ±SEM from n = 3 biologically independent animals per group; ** p < 0.01 by two-tailed unpaired Student’s t test. (B) RT-qPCR for Thbs1 , Thbs2 , Thbs3 , Thbs4 , and Thbs5 mRNA in the right tibialis anterior (TA) of 12-week-old wild-type (WT) mice subjected to 3 or 6 days (d) of unilateral hindlimb denervation, compared contralateral sham-operated leg. Error denote ±SEM from n = 3–5 biologically independent animals per group. * p < 0.05 by two-tailed unpaired Student’s t test. (C) Western blot for Thbs1 and Gapdh control in right TA of 12-week-old WT mice subjected to 3 or 10 days (d) of unilateral hindlimb denervation, compared to contralateral, sham-operated TA. n = 2 biologically independent animals per time point. (D) Representative immunohistochemistry for endogenous Thbs1 (green) and BiP (red) to visualize the endoplasmic reticulum (ER) on cryo-embedded TA of WT mice subjected to 10 days (d) of denervation compared to sham-operated controls at 12 weeks of age. Nuclei are shown in blue with DAPI. Scale bars represent 50 μm. (E) RT-qPCR for Thbs1 mRNA from TA of 8-week-old mice fed ad libitum or fasted for 48 h. Data are presented as fold expression over fed WT; error bars denote ±SEM from n = 4 biologically independent animals analyzed per group. * p < 0.05 by two-tailed unpaired Student’s t test. (F and G) Western blot for Thbs1 and Gapdh in TA of 8-week-old mice fed ad libitum or fasted for 48 h (F), and in young (12 weeks of age) and old (24 months of age) WT quadriceps (G). (H) Representative immunohistochemistry for endogenous Thbs1 (green), BiP (red), and the nucleus (DAPI, blue) on cryo-embedded 12-week-old (“young”) and 24-month-old (“old”) WT quadriceps. Scale bars represent 50 μm.

Article Snippet: A literature search yielded a publicly available Affymetrix Mouse Genome 430A 2.0 microarray dataset (NCBI GEO Repository, GSE80223) in which Gambara G. et al. compared the gene expression adaptation in soleus from adult C57Bl/n6 mice that were flown in space aboard the BION-M1 biosatelite for 30 days in orbit ( n = 3), and from sex- and age-matched control mice that were housed in standard vivarium cages ( n = 3).

Techniques: Expressing, Control, Microarray, Two Tailed Test, Quantitative RT-PCR, Western Blot, Immunohistochemistry

Journal: Cell reports

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner

doi: 10.1016/j.celrep.2024.114149

Figure Lengend Snippet:

Article Snippet: A literature search yielded a publicly available Affymetrix Mouse Genome 430A 2.0 microarray dataset (NCBI GEO Repository, GSE80223) in which Gambara G. et al. compared the gene expression adaptation in soleus from adult C57Bl/n6 mice that were flown in space aboard the BION-M1 biosatelite for 30 days in orbit ( n = 3), and from sex- and age-matched control mice that were housed in standard vivarium cages ( n = 3).

Techniques: Virus, Recombinant, Electron Microscopy, Plasmid Preparation, SYBR Green Assay, Membrane, Blocking Assay, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Protein Extraction, Clone Assay, Expressing, Software, Microscopy, Real-time Polymerase Chain Reaction